Human GCK ELISA Kit

Product Name:Human GCK ELISA Kit
Packing:96T

Catalog No.:ELA-E0667h

Gene Name:GCK

Detect Range:78-5000 pg/mL

Sensitivity:45pg/mL

Target Protein Name:Human Glucokinase

Alternative Name:Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, GCK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human GCK ELISA Kit allows for the in vitro quantitative determination of Human Glucokinase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human GCK ELISA Kit has been pre-coated with an GCK antibody specific to Human Glucokinase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Glucokinase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Glucokinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm