Human GDF9 ELISA Kit

Product Name:Human GDF9 ELISA Kit
Packing:96T

Catalog No.:ELA-E0427h

Gene Name:GDF9

Detect Range:0.156-10 ng/mL

Sensitivity:0.12ng/mL

Target Protein Name:Human Growth/differentiation factor 9

Alternative Name:Growth/differentiation factor 9, GDF-9, GDF9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human GDF9 ELISA Kit allows for the in vitro quantitative determination of Human Growth/differentiation factor 9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human GDF9 ELISA Kit has been pre-coated with an GDF9 antibody specific to Human Growth/differentiation factor 9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Growth/differentiation factor 9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Growth/differentiation factor 9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm