Human GINGIPAIN R ELISA Kit
Packing.96Tests Cat No. EF0074411
【INTENDED USE】
For the quantitative detection of Human Gingipain R (RGP) concentration in serum, plasma and other biological fluids.
【INTRODUCTION】
RGP is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been 4 characterized. RGP is a chemical signal produced by cells that stimulates the growth of new blood vessels. The most important member is RGP-A. Other members are Placenta growth factor (PlGF), RGP-B, RGP-C and RGP-D. The latter ones were discovered later than RGP-A, and, before their discovery, RGP-A was called just RGP.
【PRINCIPLE OF THE ASSAY】
This assay employs a two-site sandwich ELISA to quantitate GINGIPAIN R in Human serum, plasma. An antibody specific for GINGIPAIN R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GINGIPAIN R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GINGIPAIN R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GINGIPAIN R bound in the initial step. The color development is stopped and the intensity of the color is measured.
【LIMITATIONS OF THE PROCEDURE】
※ FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
※ The kit should not be used beyond the expiration date on the kit label.
※ Do not mix or substitute reagents with those from other lots or sources.
※ It is important that the Calibrator Diluent selected for the standard curve be
consistent with the samples being assayed.
※ If samples generate values higher than the highest standard, dilute the samples with the appropriate Calibrator Diluent and repeat the assay.
※ Any variation in standard diluent, operator, pipetting technique, washing
technique, incubation time or temperature, and kit age can cause variation in binding.
※ This assay is designed to eliminate interference by soluble receptors,
binding proteins, and other factors present in biological samples. Until all factors have been tested in the ELISA Kit, the possibility of interference cannot be excluded.
【DETECTION RANGE】
31.25 pg/mL - 2000 pg/mL. The standard curve concentrations used for the ELISA’s were 2000 pg/mL, 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL,
31.25 pg/mL, 0 pg/mL.
【SENSITIVITY】
The limit of detection of Human GINGIPAIN R defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 10.4 pg/mL (mean of 6 independent assays).
【SPECIFICITY】
This assay has high sensitivity and excellent specificity for detection of Human GINGIPAIN R. No significant cross-reactivity or interference between Human GINGIPAIN R and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Human GINGIPAIN R and all the analogues, therefore, cross reaction may still exist.
【PRECISION】
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8%
Inter-Assay: CV<12%
【STABILITY】
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2-8°C, which means 7 days at 37°C equaling 12 months at 2-8°C).
Note:
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
This package insert must be read in its entirety before using this product.
Caution:
Product is for research use only!