Human glucocorticoid ELISA Kit
Product Name:Human glucocorticoid ELISA Kit
Packing:96T
Catalog No.:ELA-E0941h
Gene Name:glucocorticoid
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:glucocorticoid
Alternative Name:glucocorticoid,glucocorticoid
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human glucocorticoid ELISA Kit allows for the in vitro quantitative determination of glucocorticoid concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human glucocorticoid ELISA Kit has been pre-coated with an glucocorticoid antibody specific to glucocorticoid .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for glucocorticoid and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain glucocorticoid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of glucocorticoid in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!