Human Glutamic Acid Decarboxylase Autoantibody, GAD-Ab ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Glutamic Acid Decarboxylase Autoantibody,GAD-Ab, Anti-GAD,anti Glutamic Acid Decarboxylase,Anti GAD,anti Glutamate decarboxylase,

Search name

Human Glutamic Acid Decarboxylase Autoantibody ELISA KIT ,Human GAD-Ab ELISA KIT ,Human Anti-GAD ELISA KIT ,Human anti Glutamic Acid Decarboxylase ELISA KIT ,Human Anti GAD ELISA KIT ,Human anti Glutamate decarboxylase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Glutamic Acid Decarboxylase Autoantibody,GAD-Ab concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen specific to GAD-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen preparation specific for GAD-Ab and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GAD-Ab, biotin-conjugated antigen and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GAD-Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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