Human Glutathione S- transferase P, GSTP1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glutathione S-transferase P,GST class-pi,GSTP1-1,GSTP1,GSTP1-1; DFN7; FAEES3; GST3; GSTP; HEL-S-22; GST class-pi; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; glutathione S-transferase P; glutathione S-transferase pi 1, P,GST class-pi,Chain 7,GST 7-7,
Search name
Human Glutathione S-transferase P ELISA KIT ,Human GST class-pi ELISA KIT ,Human GSTP1-1 ELISA KIT ,Human GSTP1 ELISA KIT ,Human GSTP1-1 ELISA KIT ,Human DFN7 ELISA KIT ,Human FAEES3 ELISA KIT ,Human GST3 ELISA KIT ,Human GSTP ELISA KIT ,Human HEL-S-22 ELISA KIT ,Human GST class-pi ELISA KIT ,Human deafness ELISA KIT ,Human X-linked 7 ELISA KIT ,Human epididymis secretory protein Li 22 ELISA KIT ,Human fatty acid ethyl ester synthase III ELISA KIT ,Human glutathione S-transferase P ELISA KIT ,Human glutathione S-transferase pi 1 ELISA KIT ,Human P ELISA KIT ,Human GST class-pi ELISA KIT ,Human Chain 7 ELISA KIT ,Human GST 7-7 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Glutathione S-transferase P concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S-transferase P, During the reaction, Glutathione S-transferase P in the sample or standard competes with a fixed amount of biotin-labeled Glutathione S-transferase P for sites on a pre-coated Monoclonal antibody specific to Glutathione S-transferase P. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glutathione S-transferase P in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download