Gp130/IL6ST ELISA KIT|Human
Catalog EF000126
Packing 48T/96T
Reactivity Human
Detect Range 0.25-16ng/ml
Gp130/IL6ST ELISA KIT Sensitivity< 0.15ng/ml
Application Gp130/IL6ST ELISA KIT|Human is for detecting the concentration of Human Gp130/IL6ST in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Gp130/IL6ST ELISA KIT|Human
The microtiter plate provided in Gp130/IL6ST ELISA KIT|Human has been pre-coated with an Human Gp130/IL6ST antibody specific to Human Gp130/IL6ST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Gp130/IL6STantibody preparation specific for Human Gp130/IL6ST then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Gp130/IL6ST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Gp130/IL6STin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Gp130/IL6ST ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!