Human Grainyhead- like protein 3 homolog, GRHL3 ELISA KIT
Product Name:Human Grainyhead- like protein 3 homolog, GRHL3 ELISA KIT
Packing:96T
Catalog No.:ELI-08718h
Gene Name:GRHL3
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Grainyhead-like protein 3 homolog(GRHL3
Alternative Name:GRHL3,Grainyhead-like protein 3 homolog(GRHL3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Grainyhead- like protein 3 homolog, GRHL3 ELISA KIT allows for the in vitro quantitative determination of Grainyhead-like protein 3 homolog(GRHL3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Grainyhead- like protein 3 homolog, GRHL3 ELISA KIT has been pre-coated with an Grainyhead-like protein 3 homolog(GRHL3 antibody specific to Grainyhead-like protein 3 homolog(GRHL3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Grainyhead-like protein 3 homolog(GRHL3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Grainyhead-like protein 3 homolog(GRHL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Grainyhead-like protein 3 homolog(GRHL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!