Human Group XIIA secretory phospholipase A2, PLA2G12A ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PLA2G12A,GXII; PLA2G12; ROSSY; GXII sPLA2; group XII secreted phospholipase A2; group XIIA secreted phospholipase A2; group XIIA secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 12A; phospholipase A2, group XII; sPLA2-XII; phospholipase A2, group XIIA

Search name

Human PLA2G12A ELISA KIT ,Human GXII ELISA KIT ,Human PLA2G12 ELISA KIT ,Human ROSSY ELISA KIT ,Human GXII sPLA2 ELISA KIT ,Human group XII secreted phospholipase A2 ELISA KIT ,Human group XIIA secreted phospholipase A2 ELISA KIT ,Human group XIIA secretory phospholipase A2 ELISA KIT ,Human phosphatidylcholine 2-acylhydrolase 12A ELISA KIT ,Human phospholipase A2 ELISA KIT ,Human  group XII ELISA KIT ,Human sPLA2-XII ELISA KIT ,Human phospholipase A2 group XIIA ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Group XIIA secretory phospholipase A2, PLA2G12A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Group XIIA secretory phospholipase A2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Group XIIA secretory phospholipase A2 and Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Group XIIA secretory phospholipase A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Group XIIA secretory phospholipase A2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product

Delivery time 4-6month