Human Growth factor receptor- bound protein 2, GRB2 ELISA KIT
Product Name:Human Growth factor receptor- bound protein 2, GRB2 ELISA KIT
Packing:96T
Catalog No.:ELI-39084h
Gene Name:GRB2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Growth factor receptor-bound protein 2(GRB2
Alternative Name:GRB2,Growth factor receptor-bound protein 2(GRB2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Growth factor receptor- bound protein 2, GRB2 ELISA KIT allows for the in vitro quantitative determination of Growth factor receptor-bound protein 2(GRB2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Growth factor receptor- bound protein 2, GRB2 ELISA KIT has been pre-coated with an Growth factor receptor-bound protein 2(GRB2 antibody specific to Growth factor receptor-bound protein 2(GRB2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Growth factor receptor-bound protein 2(GRB2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Growth factor receptor-bound protein 2(GRB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Growth factor receptor-bound protein 2(GRB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!