Human Guanylin, GUCA2A ELISA KIT

Product Name:Human Guanylin, GUCA2A ELISA KIT
Packing:96T

Catalog No.:ELI-08474h

Gene Name:GUCA2A

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Guanylin(GUCA2A

Alternative Name:GUCA2A,Guanylin(GUCA2A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Guanylin, GUCA2A ELISA KIT allows for the in vitro quantitative determination of Guanylin(GUCA2A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Guanylin, GUCA2A ELISA KIT has been pre-coated with an Guanylin(GUCA2A antibody specific to Guanylin(GUCA2A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Guanylin(GUCA2A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Guanylin(GUCA2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Guanylin(GUCA2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!