Human H/ACA ribonucleoprotein complex subunit 4, DKC1 ELISA KIT
Product Name:Human H/ACA ribonucleoprotein complex subunit 4, DKC1 ELISA KIT
Packing:96T
Catalog No.:ELI-08279h
Gene Name:DKC1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:H(ACA ribonucleoprotein complex subunit 4(DKC1
Alternative Name:DKC1,H(ACA ribonucleoprotein complex subunit 4(DKC1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human H/ACA ribonucleoprotein complex subunit 4, DKC1 ELISA KIT allows for the in vitro quantitative determination of H(ACA ribonucleoprotein complex subunit 4(DKC1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human H/ACA ribonucleoprotein complex subunit 4, DKC1 ELISA KIT has been pre-coated with an H(ACA ribonucleoprotein complex subunit 4(DKC1 antibody specific to H(ACA ribonucleoprotein complex subunit 4(DKC1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for H(ACA ribonucleoprotein complex subunit 4(DKC1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain H(ACA ribonucleoprotein complex subunit 4(DKC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of H(ACA ribonucleoprotein complex subunit 4(DKC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!