Human HCRTR1 ELISA Kit

Product Name:Human HCRTR1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0984h

Gene Name:HCRTR1

Detect Range:78-5000 pg/mL

Sensitivity:34pg/mL

Target Protein Name:Human Orexin receptor type 1

Alternative Name:Orexin receptor type 1, Ox-1-R, Ox1-R, Ox1R, Hypocretin receptor type 1, HCRTR1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human HCRTR1 ELISA Kit allows for the in vitro quantitative determination of Human Orexin receptor type 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human HCRTR1 ELISA Kit has been pre-coated with an HCRTR1 antibody specific to Human Orexin receptor type 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Orexin receptor type 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Orexin receptor type 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm