Human Hepatoma-derived growth factor-related protein 3, HDGFRP3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

HDGFRP3,CGI-142; HDGF-2; HDGF2; HRP-3; hepatoma-derived growth factor 2; hepatoma-derived growth factor-related protein 3; hepatoma-derived growth factor related protein 3

Search name

Human HDGFRP3 ELISA KIT ,Human CGI-142 ELISA KIT ,Human HDGF-2 ELISA KIT ,Human HDGF2 ELISA KIT ,Human HRP-3 ELISA KIT ,Human hepatoma-derived growth factor 2 ELISA KIT ,Human hepatoma-derived growth factor-related protein 3 ELISA KIT ,Human hepatoma-derived growth factor related protein 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human HRP-3 concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HRP-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHRP-3 and Avidin conjugated to Horseradish Peroxidase(HRP) is added to each microplate well and incubated. Then a TMB su002276bstrate solutioWn is added to each well. Only those wells that contain HRP-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HRP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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