Human Heat shock 70 kDa protein 12B, HSPA12B ELISA KIT

Product Name:Human Heat shock 70 kDa protein 12B, HSPA12B ELISA KIT
Packing:96T

Catalog No.:ELI-27127h

Gene Name:HSPA12B

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Heat shock 70 kDa protein 12B(HSPA12B

Alternative Name:HSPA12B,Heat shock 70 kDa protein 12B(HSPA12B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Heat shock 70 kDa protein 12B, HSPA12B ELISA KIT allows for the in vitro quantitative determination of Heat shock 70 kDa protein 12B(HSPA12B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Heat shock 70 kDa protein 12B, HSPA12B ELISA KIT has been pre-coated with an Heat shock 70 kDa protein 12B(HSPA12B antibody specific to Heat shock 70 kDa protein 12B(HSPA12B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Heat shock 70 kDa protein 12B(HSPA12B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heat shock 70 kDa protein 12B(HSPA12B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Heat shock 70 kDa protein 12B(HSPA12B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!