Human Helicase SKI2W, SKIV2L ELISA KIT
Product Name:Human Helicase SKI2W, SKIV2L ELISA KIT
Packing:96T
Catalog No.:ELI-41521h
Gene Name:SKIV2L
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Helicase SKI2W(SKIV2L
Alternative Name:SKIV2L,Helicase SKI2W(SKIV2L
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Helicase SKI2W, SKIV2L ELISA KIT allows for the in vitro quantitative determination of Helicase SKI2W(SKIV2L concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Helicase SKI2W, SKIV2L ELISA KIT has been pre-coated with an Helicase SKI2W(SKIV2L antibody specific to Helicase SKI2W(SKIV2L .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Helicase SKI2W(SKIV2L and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Helicase SKI2W(SKIV2L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Helicase SKI2W(SKIV2L in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!