Human Hemolytic complement ELISA Kit

Product Name:Human Hemolytic complement ELISA Kit
Packing:96T

Catalog No.:ELA-E1182h

Gene Name:Hc

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Hemolytic complement

Alternative Name:Hc,Hemolytic complement
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Hemolytic complement ELISA Kit allows for the in vitro quantitative determination of Hemolytic complement concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Hemolytic complement ELISA Kit has been pre-coated with an Hemolytic complement antibody specific to Hemolytic complement .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Hemolytic complement and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hemolytic complement , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Hemolytic complement in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!