Human HFE2 ELISA Kit
Product Name:Human HFE2 ELISA Kit
Packing:96T
Catalog No.:ELA-E1077h
Gene Name:HFE2
Detect Range:0.78-50 ng/mL
Sensitivity:0.22ng/mL
Target Protein Name:Human Hemojuvelin
Alternative Name:Hemojuvelin, Hemochromatosis type 2 protein, RGM domain family member C, HFE2, HJV, RGMC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human HFE2 ELISA Kit allows for the in vitro quantitative determination of Human Hemojuvelin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human HFE2 ELISA Kit has been pre-coated with an HFE2 antibody specific to Human Hemojuvelin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Hemojuvelin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Hemojuvelin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm