Human HIF1A ELISA Kit
Product Name:Human HIF1A ELISA Kit
Packing:96T
Catalog No.:ELA-E0798h
Gene Name:HIF1A
Detect Range:0.156-10 ng/mL
Sensitivity:0.041 ng/mL
Target Protein Name:Human Hypoxia-inducible factor 1-alpha
Alternative Name:Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human HIF1A ELISA Kit allows for the in vitro quantitative determination of Human Hypoxia-inducible factor 1-alpha concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human HIF1A ELISA Kit has been pre-coated with an HIF1A antibody specific to Human Hypoxia-inducible factor 1-alpha .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Hypoxia-inducible factor 1-alpha and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Hypoxia-inducible factor 1-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm