Human Histone H2A type 1- B/E, HIST1H2AB ELISA KIT

Product Name:Human Histone H2A type 1- B/E, HIST1H2AB ELISA KIT
Packing:96T

Catalog No.:ELI-08481h

Gene Name:HIST1H2AB

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Histone H2A type 1-B(E(HIST1H2AB

Alternative Name:HIST1H2AB,Histone H2A type 1-B(E(HIST1H2AB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Histone H2A type 1- B/E, HIST1H2AB ELISA KIT allows for the in vitro quantitative determination of Histone H2A type 1-B(E(HIST1H2AB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Histone H2A type 1- B/E, HIST1H2AB ELISA KIT has been pre-coated with an Histone H2A type 1-B(E(HIST1H2AB antibody specific to Histone H2A type 1-B(E(HIST1H2AB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histone H2A type 1-B(E(HIST1H2AB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone H2A type 1-B(E(HIST1H2AB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histone H2A type 1-B(E(HIST1H2AB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!