Human HLA-DRB5 ELISA Kit

Product Name:Human HLA-DRB5 ELISA Kit
Packing:96T

Catalog No.:ELA-E15038h

Gene Name:HLA-DRB5

Detect Range:1.56-100 ng/mL

Sensitivity:0.16ng/ml

Target Protein Name:Human HLA class II histocompatibility antigen, DR beta 5 chain

Alternative Name:HLA class II histocompatibility antigen, DR beta 5 chain, DR beta-5, DR2-beta-2, Dw2, MHC class II antigen DRB5, HLA-DRB5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human HLA-DRB5 ELISA Kit allows for the in vitro quantitative determination of Human HLA class II histocompatibility antigen, DR beta 5 chain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human HLA-DRB5 ELISA Kit has been pre-coated with an HLA-DRB5 antibody specific to Human HLA class II histocompatibility antigen, DR beta 5 chain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human HLA class II histocompatibility antigen, DR beta 5 chain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human HLA class II histocompatibility antigen, DR beta 5 chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human HLA class II histocompatibility antigen, DR beta 5 chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!