Human HMGB1 ELISA Kit
Product Name:Human HMGB1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0399h
Gene Name:HMGB1
Detect Range:0.156-10 ng/mL
Sensitivity:0.1 ng/mL
Target Protein Name:Human High mobility group protein B1
Alternative Name:High mobility group protein B1, High mobility group protein 1, HMG-1, HMGB1, HMG1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human HMGB1 ELISA Kit allows for the in vitro quantitative determination of Human High mobility group protein B1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human HMGB1 ELISA Kit has been pre-coated with an HMGB1 antibody specific to Human High mobility group protein B1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human High mobility group protein B1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human High mobility group protein B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm