HMGB1 ELISA KIT|Human

Catalog  EF000598

Packing 48T/96T

Reactivity Human

Detect Range 31.25-2000pg/ml

HMGB1 ELISA KIT Sensitivity< 18.75pg/ml

Application HMGB1 ELISA KIT|Human  is for detecting the concentration of Human HMGB1 in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of HMGB1 ELISA KIT|Human

The microtiter plate provided in HMGB1 ELISA KIT|Human has been pre-coated with an Human HMGB1 antibody specific to Human HMGB1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human HMGB1antibody preparation specific for Human HMGB1 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human HMGB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human HMGB1in the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that HMGB1 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!