Human IFI44 ELISA Kit
Product Name:Human IFI44 ELISA Kit
Detect Range:0.78-50 ng/mL
Target Protein Name:Human Interferon-induced protein 44
Alternative Name:Interferon-induced protein 44, p44, Microtubule-associated protein 44, IFI44, MTAP44
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human IFI44 ELISA Kit allows for the in vitro quantitative determination of Human Interferon-induced protein 44 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human IFI44 ELISA Kit has been pre-coated with an IFI44 antibody specific to Human Interferon-induced protein 44 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Interferon-induced protein 44 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Interferon-induced protein 44, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Interferon-induced protein 44 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!