Human IFT80 ELISA KIT
Product Name:Human IFT80 ELISA KIT
Packing:96T
Catalog No.:ELI-43824h
Gene Name:IFT80
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Human IFT80
Alternative Name:IFT80,Human IFT80
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human IFT80 ELISA KIT allows for the in vitro quantitative determination of Human IFT80 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human IFT80 ELISA KIT has been pre-coated with an Human IFT80 antibody specific to Human IFT80 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human IFT80 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human IFT80, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human IFT80 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!