Human IGFBP3 ELISA Kit
Product Name:Human IGFBP3 ELISA Kit
Packing:96T
Catalog No.:ELA-E0054h
Gene Name:IGFBP3
Detect Range:125-8000 pg/mL
Sensitivity:50pg/mL
Target Protein Name:Human Insulin-like growth factor-binding protein 3
Alternative Name:Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3, IGFBP3, IBP3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human IGFBP3 ELISA Kit allows for the in vitro quantitative determination of Human Insulin-like growth factor-binding protein 3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human IGFBP3 ELISA Kit has been pre-coated with an IGFBP3 antibody specific to Human Insulin-like growth factor-binding protein 3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Insulin-like growth factor-binding protein 3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Insulin-like growth factor-binding protein 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm