Human IGFBP4 ELISA Kit

Product Name:Human IGFBP4 ELISA Kit
Packing:96T

Catalog No.:ELA-E0055h

Gene Name:IGFBP4

Detect Range:0.312-20 ng/mL

Sensitivity:0.11ng/mL

Target Protein Name:Human Insulin-like growth factor-binding protein 4

Alternative Name:Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human IGFBP4 ELISA Kit allows for the in vitro quantitative determination of Human Insulin-like growth factor-binding protein 4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human IGFBP4 ELISA Kit has been pre-coated with an IGFBP4 antibody specific to Human Insulin-like growth factor-binding protein 4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Insulin-like growth factor-binding protein 4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Insulin-like growth factor-binding protein 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm