Human IK ELISA Kit

Product Name:Human IK ELISA Kit
Packing:96T

Catalog No.:ELA-E8975h

Gene Name:IK

Detect Range:31.2-2000 pg/mL

Sensitivity:10pg/mL

Target Protein Name:Human Protein Red

Alternative Name:Protein Red, Cytokine IK, IK factor, Protein RER, IK, RED, RER
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human IK ELISA Kit allows for the in vitro quantitative determination of Human Protein Red concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human IK ELISA Kit has been pre-coated with an IK antibody specific to Human Protein Red .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Protein Red and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein Red, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Protein Red in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!