Human IL3 ELISA Kit
Product Name:Human IL3 ELISA Kit
Packing:96T
Catalog No.:ELA-E0076h
Gene Name:IL3
Detect Range:15.6-1000 pg/mL
Sensitivity:5.6pg/mL
Target Protein Name:Human Interleukin-3
Alternative Name:Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor, IL3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human IL3 ELISA Kit allows for the in vitro quantitative determination of Human Interleukin-3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human IL3 ELISA Kit has been pre-coated with an IL3 antibody specific to Human Interleukin-3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Interleukin-3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Interleukin-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm