Human IL33 ELISA Kit
Product Name:Human IL33 ELISA Kit
Packing:96T
Catalog No.:ELA-E1980h
Gene Name:IL33
Detect Range:15.6-1000 pg/mL
Sensitivity:10pg/mL
Target Protein Name:Human Interleukin-33
Alternative Name:Interleukin-33, IL-33, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV, IL33, C9orf26, IL1F11, NFHEV
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human IL33 ELISA Kit allows for the in vitro quantitative determination of Human Interleukin-33 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human IL33 ELISA Kit has been pre-coated with an IL33 antibody specific to Human Interleukin-33 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Interleukin-33 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Interleukin-33, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Interleukin-33 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!