Human IL3RA ELISA Kit

Product Name:Human IL3RA ELISA Kit
Packing:96T

Catalog No.:ELA-E9249h

Gene Name:IL3RA

Detect Range:31.2-2000 pg/mL

Sensitivity:12.3pg/mL

Target Protein Name:Human Interleukin-3 receptor subunit alpha

Alternative Name:Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, IL3RA, IL3R, CD123
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human IL3RA ELISA Kit allows for the in vitro quantitative determination of Human Interleukin-3 receptor subunit alpha concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human IL3RA ELISA Kit has been pre-coated with an IL3RA antibody specific to Human Interleukin-3 receptor subunit alpha .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Interleukin-3 receptor subunit alpha and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Interleukin-3 receptor subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Interleukin-3 receptor subunit alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!