Human Inactive phospholipase D5, PLD5 ELISA KIT

Product Name:Human Inactive phospholipase D5, PLD5 ELISA KIT
Packing:96T

Catalog No.:ELI-21617h

Gene Name:PLD5

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Inactive phospholipase D5(PLD5

Alternative Name:PLD5,Inactive phospholipase D5(PLD5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Inactive phospholipase D5, PLD5 ELISA KIT allows for the in vitro quantitative determination of Inactive phospholipase D5(PLD5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Inactive phospholipase D5, PLD5 ELISA KIT has been pre-coated with an Inactive phospholipase D5(PLD5 antibody specific to Inactive phospholipase D5(PLD5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Inactive phospholipase D5(PLD5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inactive phospholipase D5(PLD5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Inactive phospholipase D5(PLD5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!