Human Inner nuclear membrane protein Man1, LEMD3 ELISA KIT
Product Name:Human Inner nuclear membrane protein Man1, LEMD3 ELISA KIT
Packing:96T
Catalog No.:ELI-19656h
Gene Name:LEMD3
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Inner nuclear membrane protein Man1(LEMD3
Alternative Name:LEMD3,Inner nuclear membrane protein Man1(LEMD3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Inner nuclear membrane protein Man1, LEMD3 ELISA KIT allows for the in vitro quantitative determination of Inner nuclear membrane protein Man1(LEMD3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Inner nuclear membrane protein Man1, LEMD3 ELISA KIT has been pre-coated with an Inner nuclear membrane protein Man1(LEMD3 antibody specific to Inner nuclear membrane protein Man1(LEMD3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Inner nuclear membrane protein Man1(LEMD3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inner nuclear membrane protein Man1(LEMD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Inner nuclear membrane protein Man1(LEMD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!