Human INPP5J ELISA KIT

Product Name:Human INPP5J ELISA KIT
Packing:96T

Catalog No.:ELI-22723h

Gene Name:INPP5J

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Human INPP5J

Alternative Name:INPP5J,Human INPP5J
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human INPP5J ELISA KIT allows for the in vitro quantitative determination of Human INPP5J concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human INPP5J ELISA KIT has been pre-coated with an Human INPP5J antibody specific to Human INPP5J .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human INPP5J and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human INPP5J, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human INPP5J in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!