Human Integrin alpha- D, ITGAD ELISA KIT

Product Name:Human Integrin alpha- D, ITGAD ELISA KIT
Packing:96T

Catalog No.:ELI-38469h

Gene Name:ITGAD

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Integrin alpha-D(ITGAD

Alternative Name:ITGAD,Integrin alpha-D(ITGAD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Integrin alpha- D, ITGAD ELISA KIT allows for the in vitro quantitative determination of Integrin alpha-D(ITGAD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Integrin alpha- D, ITGAD ELISA KIT has been pre-coated with an Integrin alpha-D(ITGAD antibody specific to Integrin alpha-D(ITGAD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Integrin alpha-D(ITGAD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Integrin alpha-D(ITGAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Integrin alpha-D(ITGAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!