Human Integrin beta- 5, ITGB5 ELISA KIT
Product Name:Human Integrin beta- 5, ITGB5 ELISA KIT
Packing:96T
Catalog No.:ELI-12844h
Gene Name:ITGB5
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Integrin beta-5(ITGB5
Alternative Name:ITGB5,Integrin beta-5(ITGB5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Integrin beta- 5, ITGB5 ELISA KIT allows for the in vitro quantitative determination of Integrin beta-5(ITGB5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Integrin beta- 5, ITGB5 ELISA KIT has been pre-coated with an Integrin beta-5(ITGB5 antibody specific to Integrin beta-5(ITGB5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Integrin beta-5(ITGB5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Integrin beta-5(ITGB5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Integrin beta-5(ITGB5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!