Human Interferon-induced 35 kDa protein, Ifi35 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Interferon-induced 35 kDa protein homolog,IFP 35,Ifi-35, IFI35,IFP35; IFP 35; ifi-35; interferon-induced 35 kDa protein; interferon-induced protein 35
Search name
Human Interferon-induced 35 kDa protein homolog ELISA KIT ,Human IFP 35 ELISA KIT ,Human Ifi-35 ELISA KIT ,Human IFI35 ELISA KIT ,Human IFP35 ELISA KIT ,Human IFP 35 ELISA KIT ,Human ifi-35 ELISA KIT ,Human interferon-induced 35 kDa protein ELISA KIT ,Human interferon-induced protein 35 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human interferon-induced 35 kda protein,Ifi-35 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ifi-35. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ifi-35 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ifi-35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ifi-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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