Human Interferon regulatory factor 4, IRF4 ELISA KIT
Product Name:Human Interferon regulatory factor 4, IRF4 ELISA KIT
Packing:96T
Catalog No.:ELI-43432h
Gene Name:IRF4
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Interferon regulatory factor 4(IRF4
Alternative Name:IRF4,Interferon regulatory factor 4(IRF4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Interferon regulatory factor 4, IRF4 ELISA KIT allows for the in vitro quantitative determination of Interferon regulatory factor 4(IRF4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Interferon regulatory factor 4, IRF4 ELISA KIT has been pre-coated with an Interferon regulatory factor 4(IRF4 antibody specific to Interferon regulatory factor 4(IRF4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Interferon regulatory factor 4(IRF4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Interferon regulatory factor 4(IRF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Interferon regulatory factor 4(IRF4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!