Human ITGA4 ELISA Kit
Product Name:Human ITGA4 ELISA Kit
Packing:96T
Catalog No.:ELA-E1511h
Gene Name:ITGA4
Detect Range:0.156-10 ng/mL
Sensitivity:0.078 ng/mL
Target Protein Name:Human Integrin alpha-4
Alternative Name:Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha, ITGA4, CD49D, CD49d
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ITGA4 ELISA Kit allows for the in vitro quantitative determination of Human Integrin alpha-4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ITGA4 ELISA Kit has been pre-coated with an ITGA4 antibody specific to Human Integrin alpha-4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Integrin alpha-4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Integrin alpha-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm