Human Keratin 16 ELISA Kit

Product Name:Human Keratin 16 ELISA Kit
Packing:96T

Catalog No.:ELA-E8852h

Gene Name:KRT16

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Keratin 16

Alternative Name:KRT16,Keratin 16
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Keratin 16 ELISA Kit allows for the in vitro quantitative determination of Keratin 16 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Keratin 16 ELISA Kit has been pre-coated with an Keratin 16 antibody specific to Keratin 16 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Keratin 16 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Keratin 16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Keratin 16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!