Human KIF25-AS1 ELISA KIT

Product Name:Human KIF25-AS1 ELISA KIT
Packing:96T

Catalog No.:ELI-47842h

Gene Name:KIF25-AS1

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Human KIF25-AS1

Alternative Name:KIF25-AS1,Human KIF25-AS1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human KIF25-AS1 ELISA KIT allows for the in vitro quantitative determination of Human KIF25-AS1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human KIF25-AS1 ELISA KIT has been pre-coated with an Human KIF25-AS1 antibody specific to Human KIF25-AS1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human KIF25-AS1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human KIF25-AS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human KIF25-AS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!