Human Kinesin light chain 4, KLC4 ELISA KIT
Product Name:Human Kinesin light chain 4, KLC4 ELISA KIT
Packing:96T
Catalog No.:ELI-08697h
Gene Name:KLC4
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Kinesin light chain 4(KLC4
Alternative Name:KLC4,Kinesin light chain 4(KLC4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Kinesin light chain 4, KLC4 ELISA KIT allows for the in vitro quantitative determination of Kinesin light chain 4(KLC4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Kinesin light chain 4, KLC4 ELISA KIT has been pre-coated with an Kinesin light chain 4(KLC4 antibody specific to Kinesin light chain 4(KLC4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Kinesin light chain 4(KLC4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Kinesin light chain 4(KLC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Kinesin light chain 4(KLC4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!