Human KLK3 ELISA Kit

Product Name:Human KLK3 ELISA Kit
Packing:96T

Catalog No.:ELA-E0151h

Gene Name:KLK3

Detect Range:0.156-10 ng/mL

Sensitivity:0.078 ng/mL

Target Protein Name:Human Prostate-specific antigen

Alternative Name:Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human KLK3 ELISA Kit allows for the in vitro quantitative determination of Human Prostate-specific antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human KLK3 ELISA Kit has been pre-coated with an KLK3 antibody specific to Human Prostate-specific antigen .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Prostate-specific antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Prostate-specific antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm