Human L- asparaginase, ASRGL1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ASRGL1,ALP; ALP1; CRASH; L-asparaginase; L-asparagine amidohydrolase; asparaginase-like 1 protein; asparaginase-like protein 1; beta-aspartyl-peptidase; isoaspartyl dipeptidase; isoaspartyl peptidase/L-asparaginase; asparaginase like 1,L-asparaginase,Asparaginase-like protein 1,L-asparagine amidohydrolase,;

Search name

Human ASRGL1 ELISA KIT ,Human ALP ELISA KIT ,Human ALP1 ELISA KIT ,Human CRASH ELISA KIT ,Human L-asparaginase ELISA KIT ,Human L-asparagine amidohydrolase ELISA KIT ,Human asparaginase-like 1 protein ELISA KIT ,Human asparaginase-like protein 1 ELISA KIT ,Human beta-aspartyl-peptidase ELISA KIT ,Human isoaspartyl dipeptidase ELISA KIT ,Human isoaspartyl peptidase/L-asparaginase ELISA KIT ,Human asparaginase like 1 ELISA KIT ,Human L-asparaginase ELISA KIT ,Human Asparaginase-like protein 1 ELISA KIT ,Human L-asparagine amidohydrolase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human L-asparaginase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-asparaginase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for L-asparaginase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain L-asparaginase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of L-asparaginase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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