Human Late cornified envelope protein 1D, LCE1D ELISA KIT
Product Name:Human Late cornified envelope protein 1D, LCE1D ELISA KIT
Packing:96T
Catalog No.:ELI-23346h
Gene Name:LCE1D
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Late cornified envelope protein 1D(LCE1D
Alternative Name:LCE1D,Late cornified envelope protein 1D(LCE1D
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Late cornified envelope protein 1D, LCE1D ELISA KIT allows for the in vitro quantitative determination of Late cornified envelope protein 1D(LCE1D concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Late cornified envelope protein 1D, LCE1D ELISA KIT has been pre-coated with an Late cornified envelope protein 1D(LCE1D antibody specific to Late cornified envelope protein 1D(LCE1D .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Late cornified envelope protein 1D(LCE1D and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Late cornified envelope protein 1D(LCE1D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Late cornified envelope protein 1D(LCE1D in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!