Human LDHA ELISA Kit

Product Name:Human LDHA ELISA Kit
Packing:96T

Catalog No.:ELA-E2195h

Gene Name:LDHA

Detect Range:3.12-200 U/L

Sensitivity:0.12ng/mL

Target Protein Name:Human L-lactate dehydrogenase A chain

Alternative Name:L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA, PIG19, 1.1.1.27
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human LDHA ELISA Kit allows for the in vitro quantitative determination of Human L-lactate dehydrogenase A chain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human LDHA ELISA Kit has been pre-coated with an LDHA antibody specific to Human L-lactate dehydrogenase A chain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human L-lactate dehydrogenase A chain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human L-lactate dehydrogenase A chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human L-lactate dehydrogenase A chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!