Human Leptin receptor, LR/ Ob- R ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Leptin receptor(LEP-R),HuB219,OB receptor(OB-R), leptin receptor,CD295; LEP-R; LEPRD; OB-R; OBR; OB receptor; huB219
Search name
Human Leptin receptor ELISA Kit, Human LEP-R ELISA Kit, Human Leptin receptor ELISA Kit, Human LR ELISA Kit, Human Ob-
R ELISA Kit, Human HuB219 ELISA Kit, Human OB receptor ELISA Kit, Human CD295 ELISA Kit, Human LEPRD ELISA Kit,
Human OBR ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human leptin receptor,LEP-R concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to LEP-R. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for LEP-R and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain LEP-R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LEP-R in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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