Human Lipoxin B4, LXB4 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Lipoxin B4, Lipoxin,Lipoxins,LXB4,LX-B4
Search name
Human LXB4 ELISA Kit, Human Lipoxin ELISA Kit, Human Lipoxins ELISA Kit, Human Lipoxin B4 ELISA Kit, Human LX-B4 ELISA Kit
Intended use
Human LXB4 ELISA Kit allows for the in vitro quantitative determination of Human Lipoxin B4 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Human LXB4 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipoxin B4, During the reaction, Lipoxin B4 in the sample or standard competes with a fixed amount of biotin-labeled Lipoxin B4 for sites on a pre-coated Monoclonal antibody specific to Lipoxin B4. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Lipoxin B4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 1.56-100ng/ml
Human LXB4 ELISA Kit PDF manual download
Order it by email:sales@lifescience-market.com