Human LPO ELISA Kit

Product Name:Human LPO ELISA Kit
Packing:96T

Catalog No.:ELA-E0296h

Gene Name:LPO

Detect Range:0.78-50 ng/mL

Sensitivity:0.34ng/mL

Target Protein Name:Human Lactoperoxidase

Alternative Name:Lactoperoxidase, LPO, Salivary peroxidase, SPO, LPO, SAPX
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human LPO ELISA Kit allows for the in vitro quantitative determination of Human Lactoperoxidase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human LPO ELISA Kit has been pre-coated with an LPO antibody specific to Human Lactoperoxidase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Lactoperoxidase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Lactoperoxidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm