Human Lys- 63- specific deubiquitinase BRCC36, BRCC3 ELISA KIT
Product Name:Human Lys- 63- specific deubiquitinase BRCC36, BRCC3 ELISA KIT
Packing:96T
Catalog No.:ELI-24251h
Gene Name:BRCC3
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Lys-63-specific deubiquitinase BRCC36(BRCC3
Alternative Name:BRCC3,Lys-63-specific deubiquitinase BRCC36(BRCC3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Lys- 63- specific deubiquitinase BRCC36, BRCC3 ELISA KIT allows for the in vitro quantitative determination of Lys-63-specific deubiquitinase BRCC36(BRCC3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Lys- 63- specific deubiquitinase BRCC36, BRCC3 ELISA KIT has been pre-coated with an Lys-63-specific deubiquitinase BRCC36(BRCC3 antibody specific to Lys-63-specific deubiquitinase BRCC36(BRCC3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lys-63-specific deubiquitinase BRCC36(BRCC3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Lys-63-specific deubiquitinase BRCC36(BRCC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Lys-63-specific deubiquitinase BRCC36(BRCC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!